Semi-automatic cell harvester Esco CelCradle X ® (CCX-SAH)

I. Specification Esco CelCradle X ® (CCX-SAH) semi-automatic cell harvesting system

Features of the CelCradle X ® Cell harvester (CCX-SAH):

• Mechanical dissociation of adherent cells from the macroporous carrier of Tide Motion
• The automatic harvesting process can be set up from washing to harvesting (secretory or non-secretory products) in a self-contained, automatic manner.
• Suitable for harvesting cells from the CelCradle X® System
• Cell seed source support for TideXcell® -002 up to TideXcell®-100 . system
• Harvest viable cells within 1 to 3 hours
• More than 90% of the harvest efficiency can be achieved.

Esco CelCradle X ® (CCX-SAH) – Working principle:

• Preparation: Disconnect the CelCradle / CelCradle X bottles from the CelCradle / CelCradle X stage.
• Installation: Mount and secure the bottle in the CCX-SAH incubator and connect it to the ductwork and consumables.
• Enzymatic digestion: Inject the dissociating enzyme or equivalent solution applied to the cell line to separate the cell from the macroscopic carrier. Incubate the bottle so that the enzymes are active through the entire culture.
• Inhibitor: Inject the enzyme inhibitor into the bottle.
• Separation: Automatically shake the bottle. The speed and duration are entered into the CCX-SAH semi-automatic harvesting system.
• Harvest: Collect the solution in a collection bag. Inject the medium into the bottle.
• Repeat: Repeat 5-6 three times on average.

Application of the CelCradle X ® (CCX-SAH) cell harvesting system:

• Autologous cell therapy
• Harvesting mammalian cells
• Harvesting biomass for seed cells/seed vessels
• Harvesting biomass for 3D bioprinting
• Harvesting biomass for cell farming / lab-grown meat
• Harvest automation for thrifty or non-regulating products
• Intracellular virus harvesting.

II. Specifications semi-automatic cell harvester Esco CelCradle X ® (CCX-SAH)