Cell culture shaker Esco CelShaker™

I. Specification Esco CelShaker ™ rotary arm tilt shaker

Features of CelShaker™:

• Adjustable speed - allows adjustments on CelShaker™ depending on mobile line and application
• Vibration damper - reduces vibration and keeps shaker in place
• Timing operation - allows user to control harvest time
• High harvesting efficiency - allows for shaker harvesting of sticky cell cultures of more than 90% of cells.

Working principle of the Esco CelShaker ™ rotary arm shaker:

• Preparation
• Rinse the bottle with PBS or an equivalent solution to remove any serum that may interfere with enzyme activity for further steps.
• Enzymatic digestion: Add dissociation enzyme or equivalent solution applied to the cell line to separate the cell from the macro-carrier. Incubate the bottle so that the enzymes are active through the entire culture.
• Inhibitors
• Installation: Hold the CelCradle™ bottles firmly in the CelShaker™ cranks.
• Separation: Input speed and duration in an adherent cell culture shaker and cycle time initiation.
• Collect the solution in the collection flask. Add medium to the bottle.
• Repeat 6-7 three times but exclude the addition of media to the final harvest. Collect all cells by centrifugation.

Application of the Esco CelShaker™ shaker:

• Semi-automatic harvest from CelCradle™ cultures

II. Specifications cell culture shaker Esco CelShaker™