Cell harvesting system Esco TideXcell ® (TXLHS)

I. Specification of the Esco TideXcell ® Automatic Cell Harvester (TXLHS)

Tide motion in cell harvesting system Esco TideXcell ® (TXLHS):

Features of Esco TideXcell ® (TXLHS):

• TThe entire harvesting process can be set up from washing to harvesting cells in a self-contained, automatic cell harvester.
• Suitable for cell harvesting from 2L TideXcell ® matrix flasks to 20L matrix flasks
• Cell seed source support for TideXcell™-010 up to TideXcell™-100 systems.
• Harvest live cells within 1 to 3 hours (harvest time depends on harvest cycle).
• Up to 10 11 tiles can be recovered with over 95% survivability from a single TXLHS unit.
• Four (4) built-in peristaltic pumps (with option to add up to 3 more) with assignable functions.

II. Esco TideXcell ® (TXLHS) - Working principle

Setting:

• Mount and secure the TideXcell™ Matrix flask in the TideXcell™ Harvest System and connect the TideXcell™ matrix flask to the tubular manifolds. It will act as a conduit for the introduction of phosphate-buffered saline (PBS), enzymes (usually Tryple Select, Accumax and Collagenase among others), enzyme inhibitors (when using culture media that does not contain serum), culture medium and waste container.
• These six tubes connect to the Matrix Vessel via a manifold.

Wash:

• Wash the substrate with phosphate-buffered saline or an equivalent solution to remove serum that may inhibit enzyme activity in the following step.

Enzyme treatment:

• Immerse the substrate with a chosen enzyme to digest the cells until the cells round up to separate.

Cell separation:

• Shake the matrix jar together with the matrix to remove cells from the porous matrix.

Collect:

• Collect cells by rinsing the substrate with cell culture medium or an equivalent solution and collect cells into the harvesting tank.

III. Advantages of cell harvesting system Esco TideXcell ® (TXLHS)

• Suitable for cell harvesting from TideXcell™ 2L Matrix Vessels to 20L Matrix Vessels.
• Cell seed source support for TideXcell™-010 up to TideXcell™-100 systems.
• Harvest live animal cells within 1 to 3 hours (harvest time depends on harvest cycle).
• The entire harvesting process is done automatically in a closed system. Manual operation is also available.
• Can recover up to 1011 tiles with over 90% survivability from a THS . unit.

IV. Application of the Esco TideXcell ® cell harvester

• TideXcell ® Automated Cell Harvester (TXLHS) for adherent cell culture including but not limited to: Insect Cells, Mammal Cells, Stem Cells
• Extending biomass for seed training from 2D to 3D
• Autologous and allogeneic cell therapy
• Intracellular virus.

V. Specifications cell harvesting system Esco TideXcell ® (TXLHS)

VI. Video