Cell culture shaker Esco CelShaker™
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Cell culture shaker Esco CelShaker™ can simulate the shaking action of the human wrist, effectively removing cells from binding to macroporous carriers. This enables semi-automatic harvesting from CelCradle™ cultures (CeCradle™ and CelCradle™ X systems), replacing tedious manual extraction of bottles.
Esco CelShaker ™ - Your CelCradle™ Harvester Companion.
***The detailed technical data sheet of the device: Esco CelShaker™ Cell Culture Shaker.
I. Specification Esco CelShaker ™ rotary arm tilt shaker
Features of CelShaker™:
- Adjustable speed - allows adjustments on CelShaker™ depending on mobile line and application
- Vibration damper - reduces vibration and keeps shaker in place
- Timing operation - allows user to control harvest time
- High harvesting efficiency - allows for shaker harvesting of sticky cell cultures of more than 90% of cells.
Working principle of the Esco CelShaker ™ rotary arm shaker:
- Preparation
- Rinse the bottle with PBS or an equivalent solution to remove any serum that may interfere with enzyme activity for further steps.
- Enzymatic digestion: Add dissociation enzyme or equivalent solution applied to the cell line to separate the cell from the macro-carrier. Incubate the bottle so that the enzymes are active through the entire culture.
- Inhibitors
- Installation: Hold the CelCradle™ bottles firmly in the CelShaker™ cranks.
- Separation: Input speed and duration in an adherent cell culture shaker and cycle time initiation.
- Collect the solution in the collection flask. Add medium to the bottle.
- Repeat 6-7 three times but exclude the addition of media to the final harvest. Collect all cells by centrifugation.
Application of the Esco CelShaker™ shaker:
- Semi-automatic harvest from CelCradle™ cultures
II. Specifications cell culture shaker Esco CelShaker™
Description |
Wrist action shaker |
Capacity |
Up to 4 CelCradle™ bottles |
Mixing angle |
0-15° |
Shaking motion |
Wrist motion |
Timer |
0 - 999 min (1 min increment) |
0 - 999 min (1 min increment) |
50-450 rpm |
Electrical requirements |
220-240 VAC |
Dimension |
290 x 310 x 375 mm |
325 x 310 x 375 mm (with rotary arms) |
|
Net weight |
50 kg |
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